Abstract: Northeast India is well known for its unique practices of preparing alcoholic beverages and other
fermented foods. Traditionally prepared rice beer is thought to possess various medicinal and therapeutic properties due to the
utilization of numerous indigenous plant species during its preparation. It exhibits rich microbial diversity, whose synergistic
action converts the starchy components of rice into beneficial metabolites such as sugar, alcohol and organic acids. It is locally
believed that traditional rice beer is effective in treating worm expulsion, insomnia, body aches, inflammation and urinary issues.
The bioprospecting result re-establishes our belief in using traditional rice beer as a medicinal probiotic while retaining all of its
other sensory properties as a beer. The present review provides a comprehensive overview of traditional rice beer from
Northeast India, with special reference to the Galo tribe, an ethnic community of Arunachal Pradesh. It discusses the ingredients,
methods of preparation and associated traditional knowledge, along with the ethnobotanical aspects of starter cake preparation.
In addition, the review examines microbial diversity, fermentation dynamics and metabolite profiles across different regions.
Strategies for enhancing the shelf life of the rice beer are also discussed. Thus, with appropriate scientific interventions,
traditionally prepared rice beer can be effectively commercialized, supporting traditional knowledge systems and boosting the
economy of tribal communities.
Key words: Ethnobotany, Fermentation, Metabolites, Microbial diversity, Probiotics, Starter cake
"A half yearly International Journal of Biological Sciences Registered in U.S. ISSN Center at the Library of Congress"
Review Article
Bioprospecting of Traditional Fermented Beer of Northeast India: With Special Reference to the Galo Tribe of Arunachal Pradesh
Nyumpi Bagra, Shreya Borthakur, Plabita Das, Sudeshna Saikia, Talo Biju and Robin Doley*
PDFhttps://doi.org/10.5281/zenodo.20033558
Original Research Article
Diversity, Composition and Conservation Status of Ichthyofauna in Dibru River: an Important Habitat for Gangetic Dolphin in Assam
Priyanka Sarkar, Nazimur Rahman Talukdar, Parthankar Choudhury* and S.P. Biswas
PDFAbstract: The Dibru River, a significant tributary of the Brahmaputra, traverses the Tinsukia and Dibrugarh
districts of Assam. It serves as a vital resource for local communities, many of whom rely heavily on fishing for their livelihoods.
However, intensive fishing pressure is believed to be a major driver of declining ichthyofaunal diversity in the region. This
study aims to assess the diversity, composition, population trends, and economic significance of fish species inhabiting the
Dibru River. Data were collected through experimental fishing at three designated sites and supplemented by surveys of
fish sold nearby landing stations, including species not captured during field sampling. A total of 76 fish species were
documented, representing 51 genera, 25 families, and 9 orders. The family Cyprinidae was the most represented (16
species), followed by Danionidae (12), Bagridae (7) and Channidae (6). According to the IUCN Red List assessment, 87%
of the recorded species are classified as Least Concern (LC), 6% as Near Threatened (NT) and 3% each as Vulnerable (VU)
and Endangered (EN), with 1% listed as Data Deficient. Of the identified species, 28 are primarily consumed as food, 7
possess exclusive ornamental value, and 41 serve both culinary and decorative purposes. During the study, dolphins were
sighted across the years at different sites along the river. This comprehensive documentation of ichthyofaunal diversity is
pivotal for guiding future research and conservation efforts, particularly in safeguarding the endangered Gangetic dolphin,
which inhabits the same riverine ecosystem.
Key words: Assam, Dibru River, Fish diversity, Gangetic dolphin
https://doi.org/10.5281/zenodo.18278720
Original Research Article
Molecular Authentication of Indian Sandalwood (Santalum album) and Its Commercial Substitute Gmelina arborea Using trnH–psbA DNA Barcoding
Sabina Shirsekar* and Mahavir Gosavi
PDFAbstract: The demand for Santalum album (Indian sandalwood) has risen dramatically in recent decades, resulting
in widespread and unsustainable harvesting. Due to its scarcity and high economic value, many manufacturers and traders
substitute other timber species, such as Gmelina arborea, which may visually resemble sandalwood but differ greatly in aroma,
density, and chemical composition. Such substitutions reduce product quality and mislead consumers and also undermine
conservation initiatives aimed at protecting authentic sandalwood resources. Traditional identification methods including
morphological observation, anatomical characterization, and chemical profiling are effective for raw wood but lose reliability
when the material is processed into powders or carvings, where diagnostic features are masked or destroyed. To overcome
these limitations, molecular tools, particularly DNA fingerprinting and DNA barcoding, have emerged as powerful
alternatives for species authentication. This study used the trnH–psbA intergenic spacer to confirm the genetic identity of
S. album and to distinguish it from Gmelina arborea, a common substitute in commercial markets. DNA sequence analysis
revealed clear genetic separation between the two species, despite their similar appearance. The S. album sequences obtained
showed a 100% match with reference accessions in GenBank, validating the accuracy of the identification and the reliability
of the marker. Similarly, G. arborea sample grouped closely with published Gmelina sequences in the phylogenetic tree,
confirming their correct classification. The findings highlight the importance of integrating DNA-based identification into
standard quality assurance protocols to protect endangered S. album populations and to strengthen traceability and
transparency in the global sandalwood market.
Key words: DNA barcoding, Gmelina arborea, Sandalwood, Santalum album, Sustainable trade, trnH–psbA intergenic spacer, White wood
https://doi.org/10.5281/zenodo.18800571
Original Research Article
Incense and Insect Repellent Plants of Darjeeling Himalaya, West Bengal, India
Pratibha Khawas, Kesang Sherpa and Kishor Biswas*
PDF
Abstract: Incense is an aromatic biological substance that releases fragrant smoke or fumes when burned and used for
appealing and aesthetic purposes as well as some other use like repelling insects. Darjeeling Himalayan region is rich in
diversified flora and varied tribal communities having rich traditional knowledge. They still prefer to use their traditional
knowledge to exploit the available plant resources for religious purposes as well as making traditional incense and insect
repellent substances. Being rich in traditional knowledge system, different aspect of ethnobotany and indigenous knowledge of
different communities of this area were studied by different workers. Lack of complete, concrete and compact study on
incense and insect repellent plants of this zone and the related aspects encouraged the present dissertation which was designed
to enumerate the botanicals used as incense and insect repellent agents by the people of Darjeeling. Standard methodology for
ethnobotanical survey including field visit, market survey, visit to the religious places, interviews with monks, knowledgeable
elderly person etc were followed to collect the data. A total of 58 species belonging to 52 genera and 26 families covering dicots,
monocots and gymnosperms which played an important role in the daily life of the tribals and the locals. The study pointed
towards the threats to some of the incense and insect repellent species and recommends further detailed and more intensive
study to investigate their proper uses and potential as more effective and environment friendly incense and insect repellent
products, population structure and ecological status of those species for their protection and conservation.
Key words: Darjeeling, ethnobotany, ethnoreligious plants, incense, insect repellents, traditional knowledge
https://doi.org/10.5281/zenodo.19016184
Original Research Article
Population Distribution of Two Sympatric Sparrow Species: House Sparrow (Passer domesticus) and Tree Sparrow (Passer montanus) in Urban Areas of Bodoland Territorial Region (BTR), Assam, India
Dipika Chetry, Eliza Basumatary, Pranjit Kumar Sarma, Sujit Deka, Jatin Sarmah and Hilloljyoti Singha*
PDF
Abstract:
We studied distribution and population densities of House Sparrow (Passer domesticus) and Tree Sparrow
(Passer montanus) along urban–suburban gradients in five district Headquarter towns of the Bodoland Territorial Region
(BTR), Assam—Kokrajhar, Kajalgaon, Udalguri, Tamulpur, and Mushalpur. We used 500 × 500 sq. m grids, selecting one
point randomly in each grid. Stratified point count surveys (30 m radius, 5-minute counts) were conducted from April 2022 to
June 2024 in different seasons across Commercial Areas (CA), Residential Areas (RA), and Suburban Areas (SUA). We did
not find significant difference in population density of both the species among different seasons and two successive years as
well as between the sexes in House Sparrows. We combined the data for further analyses. Kokrajhar had the highest density
of House Sparrow in all the three habitats, and among the three habitats, the lowest density was found in suburban area in all
the district Headquarter towns. The lowest density of Tree Sparrow was found in Kajalgaon in all the three habitats, and the
highest density of Tree Sparrow was found in sub-urban area. House Sparrows were significantly more than the Tree
Sparrows in all the district Headquarter towns and in all the habitats. We found highly significant difference of the population
of both House Sparrow and Tree Sparrow across the district Headquarter towns and also among three different habitats.
Interspecific comparisons confirmed clear habitat segregation between the two species. The study provides baseline data on
sparrow populations in the urban areas of BTR, based on which population monitoring could be carried out.
Key words: Urban ecology, House Sparrow, Tree Sparrow, Habitat preference, Urban–suburban gradient
https://doi.org/10.5281/zenodo.19436419
Original Research Article
Lichen Biomass Distribution in Different Forest Stands of Kumaun Himalaya
Ritika Tamat, Pooja Bisht and Balwant Kumar*
PDF
Abstract:
Lichens are vital components of forest ecosystems, serving as indicators of environmental quality and contributing
to nutrient cycling and habitat complexity. This study assesses lichen biomass across different forest stands in the Kumaun
Himalaya, a region known for its rich biodiversity and varying ecological zones. Quantitative biomass estimation was conducted
in representative forest types, including pine (Pinus roxburghii), oak (Quercus leucotrichophora) and mixed broadleaf stands.
Standardized sampling techniques, including stratified random sampling and destructive harvesting methods, were employed
to quantify epiphytic lichen biomass. Results revealed significant variations in biomass distribution across forest types, with the
highest biomass recorded in undisturbed oak-dominated forests, followed by mixed and pine stands. Factors influencing lichen
biomass included canopy cover, bark texture, moisture availability, and anthropogenic disturbance. The study exhibits the
ecological importance of conserving native broadleaf forests to maintain lichen diversity and biomass, which in turn support
broader ecosystem functions. This study also provides a baseline for long-term monitoring of lichen dynamics in response to
climate change and forest management practices in the Himalayan region.
Key words: Lichens, biomass, forest types, altitude, Kumaun Himalaya
https://doi.org/10.5281/zenodo.20130219
Short Communication
Successful Hatching of Rescued Eggs of Two Naja Species from Assam, India
Hiranmoy Chetia* and Daoharu Baro
PDF
Abstract:
Human-snake negative interaction is common in rural Assam, particularly during the breeding season when cobras
often deposit eggs inside houses or other structures. Such encounters frequently lead to destruction of eggs, posing a threat to
local populations of ecologically important species. We report two cases of successful hatching from rescued cobra (Naja spp.)
eggs in Assam. In the first case, thirteen eggs of a Monocled Cobra (Naja kaouthia) were recovered from beneath the mud
floor of a residence in Lakhimpur district and incubated in a container lined with straw and hay. All hatched successfully after
sixteen days. The second case is from Kokrajhar district, where eleven eggs of a Spectacled Cobra (Naja naja) were rescued
from a school, and were incubated under controlled conditions (27–32 °C, 70–80% humidity), and eight of them hatched after
fourteen days. Given the paucity of such documentation from the region, the present findings offer practical and replicable
models for wildlife rescuers, forest departments, and conservation practitioners for the safe handling and management of
rescued cobra eggs.
Key words: Monocled Cobra, Spectacled Cobra, egg rescue, human-snake conflict, Assam, incubation
https://doi.org/10.5281/zenodo.18605494
